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Fig. 4. PAR-3 associates exclusively with JAMs 1 to 3. GST fusion proteins with the C-terminal cytoplasmic domains of JAM-2, JAM-3, JAM-1, ESAM, CAR, claudin-1 and claudin-5 were incubated with [35S]-methionine labelled PAR-3 constructs comprising PDZ domains 1 to 3 (PAR-3/PDZ1-3) or full length PAR-3 (PAR-3/1-1337) as described in the legend to Fig. 1. Both PAR-3 constructs efficiently associated only with JAMs 1 to 3.





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