Fig. 7. Decreased expression of ADAM12 in 3T3-L1 cells by RNAi inhibits actin cytoskeleton reorganization. 3T3-L1 cells were co-injected with ADAM12 siRNA (A,B) or a scrambled siRNA oligo (C,D) together with a control EGFP vector. Cells were analyzed for EGFP by direct fluorescence microscopy (A,C) or for F-actin by staining with rhodamine-conjugated phalloidin (B,D). Cells injected with ADAM12 siRNA (B) maintained their stress fibers (single arrow), whereas cells injected with scrambled siRNA (D) did not contain stress fibers and showed cortical actin instead (double arrows). As a control siRNA-injected, EGFP-positive cells (E) did not exhibit ADAM12 immunostaining (F), whereas noninjected cells (G) did exhibit ADAM12 immunostaining (H). Bars, 8 µm.

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