JCS00672 Movies

Files in this Data Supplement:

• Movie 1 - Time-lapse sequence showing the dynamics of tPA-GFP vesicles in a nontreated cell (120 frames, accelerated 12 times).
• Movie 2A - Time-lapse sequence showing the dynamics of Rab27a-GFP-labelled Weibel-Palade bodies in a nontreated cell (60 frames, accelerated 34 times).
• Movie 2B - . Time-lapse sequence showing the dynamics of Rab27a-GFP-labelled Weibel-Palade bodies in a nontreated cell (60 frames, accelerated 34 times).
• Movie 3A
• Movie 3B - Movies 3A,B. Rab27a-GFP-labelled Weibel-Palade bodies undergoing clockwise or anticlockwise rotations (15 frames, accelerated 34 times).
• Movie 4A
• Movie 4B - Movies 4A,B.Oscillations of Rab27a-GFP-labelled Weibel-Palade bodies (35 frames, accelerated 34 times).
• Movie 5A
• Movie 5B - Movies 5A,B.Motions of Rab27a-GFP labelled Weibel-Palade bodies on microtubules. Cells were fixed shortly after time-lapse movies were acquired and subsequently stained for a-tubulin (green, Rab27a-GFP; red, rhodamine). 120 frames in movie 5A, 25 frames in movie 5B, accelerated 34 times).