Fig. 4. Short-range motions of WPb in HUVECs. (A) Short-range diffusive motions visualized by averaging the same sequence as in Fig. 3A. Examples are shown of simple diffusion (WPb 1), directed diffusion (WPb 2) and restricted diffusion (WPb 3). The WPb x-y trajectories are given in the centre panels (x and y in µm). Three-dimensional MSD plots (right-hand panels) were fitted according to Eqs 1-3 (see Materials and Methods). The parameters deduced from the fits are: WPb 1, D=1.5 10^-4 µm²/second; WPb 2, D=1.05x10^-3 µm²/second, v=1.07x10^-2 µm/second; WPb 3, D=1.0x10^-4 µm²/second, D_cage=3.6x10^-5 µm²/second, R_cage=49 nm. Bar, 10 µm. (B) Averages of simple diffusion (n=21), directed diffusion (n=11), and restricted diffusion (n=16) 3D MSD plots.

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