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Fig. 5. Visualization of cytoskeletal elements in HUVECs by TIRF microscopy. (A) TIRF image of the actin cytoskeleton labelled with rhodaminephalloidin. Arrowheads point to focal adhesions. The diffuse staining is probably due to the actin cortex. (B) TIRF images of the microtubule cytoskeleton visualized by immunostaining of {alpha}-tubulin. Left-hand panel: cell with radially oriented microtubules; arrowheads show bright peripheral microtubules. Right-hand panel: detail of the cell periphery in another cell. (C) WPb align with microtubules. Arrowheads indicate WPb colocalizing with microtubules in two different cells. WPb preferentially accumulate at microtubule plus-ends (green, Rab27a-GFP; red, {alpha}-tubulin). Bars, 10 µm.





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