Fig. 4. Loss of Vps20p or Vta1p causes only slightly altered kinetics of -factor internalisation. Wild-type (RH1800) and vps20 (AMY174), and wild-type (AMY165) and vta1 (AMY162) cells were grown to early exponential phase and assayed for [³⁵S]-factor internalisation at 30°C. After addition of the [³⁵S]-factor, samples were taken in duplicate at various time points and washed in either phosphate buffer pH 6 (removes unbound [³⁵S]-factor) or citrate buffer pH 1 (removes bound but non-internalised [³⁵S]-factor). Shown is the internalised -factor as a percentage of the bound -factor at each time point.

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