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Fig. 4. Fate of single cardiomyocytes. One cell per grid square was labelled with
the red fluorescent tracker dye, PKH-26 (see Materials and Methods), and
cultured for 24 days prior to fixation and analysis. There were a total of 238
initial cells from three independent experiments. (A,B) Confocal micrographs
of squares where two daughter cells (A) or a binucleate cell (B) were
observed. Staining for MyHC is in green and DNA (Hoechst 33258) in blue. Scale
bar: 50 µm.
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