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Fig. 1. Lactacystin-induced accumulation of parkin in the centrosome. SH-SY5Y cells were treated without (-Lac; A-C) or with (+Lac, D-F) lactacystin, and co-stained with antibodies against the centrosome marker {gamma}-tubulin ({gamma}-tub; A,D) and parkin (PKN; B,E). Merged images (C,F) showed significant accumulation of parkin in the centrosome after lactacystin treatment (10 µM for 12 hours). The experiment was repeated three times with similar results. Scale bars: 5 µm.





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