Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells
J Cell Sci
Appelgren et al. 116 (19): 4035.
JCS00707 Supplemental Data
Files in this Data Supplement:
Movie 1
-
Movements of
Ndc80-GFP (green) and Cut12-CFP (red) during mitosis in the fission yeast
strain Hu849.
Movie 2
-
Movements of
CFP-Cnp1 (green) and Cut12-GFP (red) during mitosis in the fission yeast strain
Hu810;
Movie 3
-
This QuickTime
movie shows the movements of Ndc80-GFP (green), CFP-Cnp1 (red) and Cut12-CFP
(red) during mitosis in the fission yeast strain Hu981.
Movie 4
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Movements of the
centromere cluster and SPB (CFP-Cnp1 (green), and Cut12-GFP (red) in interphase
in the fission yeast strain Hu810 treated with 10 mg/ml TBZ. As these are merged images, the
centromere cluster appears as one yellow spot.
Movie 5
-
Movements of the
centromere cluster and SPB (CFP-Cnp1 (green), and Cut12-GFP (red) in interphase
Hu810 control cells. As these are merged images, the centromere cluster appears
as one yellow spot.
Fig S1
-
Seconvolved (z
step=0.3 mm) stacks of
wild-type, mis6 and
rik1-304 cells
subjected to immunofluorescence microscopy for Pom152-GFP nuclear periphery
marker (white) and centromere (otr) fluorescence in situ hybridization (green).
The stacks show that the declustered centromeres in mis6 mutants tend to be peripheral in
the nuclei and that the decondensed centromeres in rik1-304 mutants are peripheral in the
nuclei.
