Fig. 4. Declustering of centromeres in mis6 and nuf2-1 cells and localization of declustered centromeres to the nuclear periphery. (A) Images and statistical analysis of live cells showing the location of cen1::lacO-LacI-GFP (green) signals and the SPB marker Cut12-CFP (red) in wild-type, mis6 and nuf2-1 cells as indicated. Scale bar: 1.0 µm. cen1 at SPB indicates that cen1::lacO-LacI-GFP is within 0.2 µm from Cut12-CFP and cen1 away from SPB indicates that cen1::lacO-LacI-GFP is >0.2 µm from Cut12-CFP. (B) Images showing the location of centromeres (green) with respect to the nuclear periphery marker Pom152-GFP (white). Scale bar: 1.0 µm. See also deconvolved stacks of images (see supplemental Fig. S1 at http://jcs.biologists.org/supplemental). (C) Diagram showing the distance in one focal plane between the declustered centromere cen(otr)-FISH signal and the nuclear periphery as marked by Pom152-GFP (strains were Hu334 wild type (n=26), Hu903 rik1 (n=36) and Hu900 mis6 (n=16).

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