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Fig. 5. Effect of a low TGF-ß1 concentration on 
6 (CD49f) and ß1
(CD29) integrin expression by keratinocytes. Long-term cultures were initiated
with Adh+++EGF-R-/+ cells and performed with or without
10 pg/ml TGF-ß1. Cells at passage 4 were plated on glass slides, grown
for 72 hours, and then processed for immunofluorescence. Expression of
integrins was analyzed by Laser Scanning Cytometry (LSC) and compared in the
two culture conditions. (A) Isotypic control corresponding to 6
integrin labeling. (B) Isotypic control corresponding to ß1 integrin
labeling. (C) 6 integrin expression profile of keratinocytes grown in
the control condition. (D) ß1 integrin expression profile of
keratinocytes grown in the control condition. (E) 6 integrin expression
profile of keratinocytes grown in the presence of 10 pg/ml exogenous
TGF-ß1. (F) ß1 integrin expression profile of keratinocytes grown in
the presence of 10 pg/ml exogenous TGF-ß1. Representative labeling
profiles from a typical experiment are presented (n=3).
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