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Budding without COPII

Transport of proteins from the ER to the Golgi is relatively well understood: vesicle budding, for example, is known to require the ordered assembly of COPII coat proteins, and the SNARE proteins syntaxin 5 and BET1 are implicated in vesicle targeting. By contrast, almost nothing is known about ER-to-Golgi trafficking of the pre-chylomicron transport vesicles (PCTVs) that transport lipid. Charles Mansbach, II and co-workers have therefore developed an in vitro system for analysing PCTV trafficking, using ER isolated from labelled small intestinal mucosal cells (see p. 415). They demonstrate that PCTVs, although distinct from protein-transport vesicles, nevertheless contain COPII proteins and the two SNAREs. Surprisingly, the authors find that antibodies to the COPII protein Sar1, which block budding of protein-transport vesicles, actually stimulate PCTV budding (addition of recombinant Sar1 reverses this effect). However, they show that PCTVs that are formed in the absence of Sar1, which lack the other COPII proteins, cannot fuse with the Golgi. Mansbach and co-workers therefore suggest that, in contrast to the protein-transport vesicles, PCTVs do not require COPII proteins for budding but instead require them for fusion with the Golgi.

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COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle

Shadab A. Siddiqi, Fred S. Gorelick, James T. Mahan, and Charles M. Mansbach, II
JCS 2003 116: 415-427. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?