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Fig. 4. Localization of WAVE1-EGFP, WAVE2-EGFP and WAVE3-EGFP along the leading edge of lamellipodia and at the tips of filopodia. The growth cones were double stained with anti-GFP antibody (green) and rhodamine-phalloidin (red). The upper panel shows the growth-cone lamellipodium (A,C,E) and the lower panel shows the growth-cone microspikes and filopodia (B,D,F). The fluorescence signal emitted from each WAVE-EGFP coincides with that from anti-GFP antibody labeled with fluorescence dye. (A,B) WAVE1-GFP. (C,D) WAVE2-EGFP. (E,F) WAVE3-EGFP. Bar, 1 µm.





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