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Fig. 9. D2O-induced membrane expansion. (A) Embryos at 75 minutes post fertilization, prior to application of D2O. Asterisks indicate embryos lacking vitelline envelopes. (B) Following 10 minutes incubation in 60% D2O, devitellinated embryos increase their surface area and spread across the substrate. (C) Side view, obtained by recording down through a 45° prism in a culture dish. Frames are ~3 minutes apart.





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