Fig. 2. Schematic representation of the GFP fusions used in transient expression experiments. (a) The THI50-GFP construct corresponds to the N-terminus of THI1 (first 105 amino acids) containing the entire chloroplast transit peptide followed by 50 amino acids of the mature THI1 (which includes the putative mitochondrial presequence), with both 5' in-frame ATG codons. (b) THI501-GFP, the first ATG was mutated to ATC whereas the second in-frame start codon remained intact. (c) THI502-GFP, in which the second ATG was converted to ATC. (d) THImet2-GFP starts at the second ATG codon.

Return to article