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Fig. 3. Influence of Legionella LPSs on BMGs of LPS-responsive mice. BMGs (5x105 cells) from C3H/HeOU mice were incubated for 24 hours at 37°C with various concentrations of LPSs from B. pertussis (•), and from the L. pneumophila strains CS338 ({circ}), RC1 ({blacktriangledown}), and 811 ({blacksquare}). CD14 was detected by incubation with the anti-CD14 antibody rmC5-3 (2.5 µg/ml, 30 minutes, 0°C), and further incubation (30 minutes, 0°C) with a FITC-labeled goat anti-rat Ig mAb. The percentage of fluorescent cells was determined by FACS analysis of the gated granulocyte population.





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