Fig. 7. Analysis of the re-purified lipid A fractions of L. pneumophila RC1. A re-purified lipid A fraction (re-extracted with phenol/deoxycholate) from L. pneumophila RC1 analyzed for the presence of contaminants (A) and for its ability to induce CD14 expression in BMGs from C57BL/10ScCr (B) and TLR2^-/- (C) mice. (A) Samples (40 µg) of LPS from E. coli J5 and of re-purified lipid A from L. pneumophila RC1 were analyzed by SDS-PAGE stained with silver nitrate. (B) Western blot analysis of CD14 expression induced in BMGs from C57BL/10ScCr mice by 10 µg/ml of the same samples of crude LPS from E. coli and re-purified Lipid A from L. pneumophila RC1. (C) Western blot analysis of CD14 expression induced in BMGs from TLR2^+/+ and TLR2^-/- mice by 10 µg/ml of re-purified LPS and Lipid A from L. pneumophila RC1.

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