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Fig. 8. Inhibitory effect of B. pertussis lipid A in C3H/HeJ cells. BMGs
from C3H/HeJ mice were preincubated (90 minutes, 37°C) in the presence of
various concentrations of B. pertussis lipid A. (A) The cells were
reincubated (20 hours, 37°C) with (O) or without (•) LPS (1 µg/ml)
from L. pneumophila CS338, and CD14 was detected by FACS analysis of
the gated granulocyte population, as described in the legend to
Fig. 3. (B) The cells were
reincubated (20 hours, 37°C) with or without the lipid A fraction of
L. pneumophila (0.1 µg/ml), and CD14 expression was analysed by
western blot as described in the legend to
Fig. 1.
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