Fig. 6. Ectopically expressed SMN produces numerous SMN foci. (A) HeLa cells expressing high levels of myc-tagged SMN were visualized by anti-myc antibodies (green). Coilin was detected in the same cell with R508 (in red), and the merged image is shown (right panel). Arrows denote Cajal bodies, which contain both SMN and coilin, whereas arrowheads mark some of the SMN gems, which are present both in the cytoplasm and the nucleus. The bottom panels display some of the large SMN foci that are observed upon high expression of SMN. (B) MEF coilin-knockout cells transiently transfected with myc-SMN were detected by anti-myc antibodies. Arrowheads demarcate some of the foci, including large aggregates, formed in high expressing cells (right).

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