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Fig. 1. Characterisation of Munc18 isoforms in mast cells. (A) Some Munc18 isoforms are expressed in RBL cells. Total brain (1 µg) and RBL cell lysates (2x 105 cell equivalents) were resolved on SDS-PAGE, transferred to nitrocellulose and probed with antibodies to Munc18-1 (1/1000), Munc18-2 or Munc18-3 (all at 1 µg/ml). (B) Munc18-2 and Munc18-3 are expressed in mast cell lines or primary mast cells. Lysates of RBL, C57.1, BMMC and MCP-5/L cells were resolved on SDS-PAGE, transferred to nitrocellulose and probed with anti-Munc18-2 or Munc18-3.





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