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Fig. 2. Interaction of Munc18 isoforms with syntaxins. (A) Proteins from RBL cell lysates were immunoprecipitated with antibodies (5 µg) to syntaxin 2, 3 or 4. Immunoprecipitates were resolved on SDS-PAGE and immunoblotted with antibodies to Munc18-2 or Munc18-3 (upper panel) or with antibodies to syntaxin 2, 3 and 4 (lower panel) (all at 1 µg/ml). (B) Proteins from RBL cell lysates were immunoprecipitated with antibodies (5 µg) to Munc18-2 or GST control, resolved on SDS-PAGE and immunoblotted with antibodies to syntaxin 2, 3 and 4 (upper panel) and Munc18-2 (lower panel).





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