Fig. 1. Treatment with Daxx-specific RNAi species results in depletion of DAXX protein. Western blot analysis of whole-cell lysates prepared from RNAi treated cells. Samples in the upper panels are detected with anti-DAXX antibody, with the arrow indicating the DAXX band; samples in the lower panels are detected with anti-ß-actin antibody as a loading control. Mock transfections were performed with buffer alone. hDx1, hDx2 and mDx2 refer to RNAi species, as described in Materials and Methods. Unless otherwise specified, cell lysates were prepared from HeLa cells, and were collected 72 hours post-transfection. In B, cells were harvested 3 days (3d), 5 days (5d) or 9 days (9d) following RNAi transfection. In D, cells were transfected with mouse Daxx cDNA (+mDaxx) 24 hours following RNAi treatment. Cell lysates were prepared from 293 (E) and U2OS (F) cells following one round (1X) or two rounds (2X) of transfection.

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