Fig. 1. cPLA₂ is localized to the nuclear envelope in sub-confluent and confluent cells in response to norepinephrine (NE) and ionomycin (ION) in VSMCs. Sub-confluent (70% confluent) or confluent (100% confluent) VSMCs from rabbit aorta were stimulated with NE (10 µM), ION (1 µM) or their vehicles (V) for 10 minutes in the presence of 1.8 mM Ca²⁺. (A) cPLA₂ was detected using a monoclonal antibody specific for cPLA₂ and a biotinylated second antibody recognized by Texas-Red—streptavidin. Immunofluorescent staining was visualized by confocal microscopy (100x magnification). (B) Density of cPLA₂ fluorescence around the nuclear envelope was quantified using the NIH Image 1.62 program as described in Materials and Methods (n=5). ^*Value significantly different from the corresponding value obtained in the presence of V (P<0.05).

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