Fig. 2. Immunofluorescence analysis of hemidesmosomal components in GABEB cells. GABEB cells were fixed and immunolabeled for plectin (A), BP180 (B), BP230 (C) and ß4 (A-C). In D-I, GABEB cells were transfected with cDNA encoding wild-type BP180 tagged at the N-terminus with a FLAG-epitope (yellow square in diagrams at the bottom of D and H; diagrams represent cDNA encoding wild-type BP180) stained for BP180 (D,F,H,I), ß4 (E,F) or BP230 (G,I). Merged images (A-C,F) show plectin, BP180 and BP230 in green and ß4 in red. In the merged image I, BP180 is in green and BP230 is in red. Note that on reconstitution of BP180, BP230 is recruited into HD-like structures, whereas it is not recruited when BP180 expression is absent. Bars, 10 µm.

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