Fig. 7. Yeast two-hybrid analysis of interactions between ß4 and BP230 and ß4 and BP180. (Top) Schematic representation of the cytoplasmic domain of ß4. FNII repeats are represented by boxes in which the number of the repeats is shown. TM, transmembrane region. (Bottom) Cotransformation of yeast host strain PJ69-4A with ß4 mutants in the pACT2 (AD) vector and BP230^1-1156 or BP180^1-401 in the pAS2.1 (BD) vector. For further details see Fig. 3. Note that BP230 requires the third and fourth FNIII repeats, as well as the last 20 amino acids of the CS of ß4, to allow an interaction, whereas BP180 only requires the third FNIII repeat.

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