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Fig. 8. Binding of ß4 to plectin is essential for the efficient recruitment of BP180 and BP230 into HDs. PA-JEB keratinocytes stably expressing a ß4 mutant that is unable to bind to plectin (ß4R1281W) were immunolabeled for ß4, plectin, BP180 and BP230. In the merged images (C,F,I) plectin, BP180 and BP230 are in green, and ß4 is in red. Co-localized staining appeared in yellow. Note that prevention of the interaction between ß4 and plectin results in a diminished localization of BP180 and BP230 in HD-like structures. Bar, 10 µm.





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