Fig. 1. Expression of SKD1(E235Q) induces the accumulation of recycling receptors in E235Q compartments. MDCK cells were coinfected with adenoviruses encoding GFP-SKD1(E235Q) and LDLR (A-C). Polarized WIF-B cells were solely infected with the adenovirus encoding GFP-SKD1(E235Q) and BC indicates the bile canalicular-like space corresponding to the apical membrane in polarized cells (D-F). Note that GFP-SKD1(E235Q)-expressing cells (asterisks) had large phase-lucent vacuoles (C,F). The distribution of GFP-SKD1(E235Q) was visualized directly by GFP fluorescence (green in A,D). LDLR and ASGPR were labeled with specific antibodies (B,E) and were merged with GFP images as red (A,D). Arrows indicate the localization of LDLR (A,B) and ASGPR (D,E) in basolateral endosomes in the cells that did not express GFP-SKD1(E235Q). Arrowheads represent the accumulation of LDLR and ASGPR in E235Q compartments in SKD1(E235Q)-expressing cells. Bars, 20 µm.

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