Fig. 2. Effects of SKD1(E235Q) expression on TGN38 distribution and traffic. NRK cells were infected with the adenovirus encoding GFP-SKD1(E235Q), fixed and stained with mAb against TGN38 (A-C). Panel A shows the merged image of GFP-SKD1(E235Q) (green) and TGN38 (red). Arrows indicate the colocalization of GFP-SKD1(E235Q) with TGN38. The cell surface TGN38 was labeled with mAb and chased at 37°C for 1 hour before fixation and visualization with a Cy3-labeled secondary antibody (D-F). Panel D shows the merged image of GFP-SKD1(E235Q) (green) and internalized antibody against TGN38 (red). In uninfected cells, the internalized antibodies reach the TGN (arrowheads in D,E), while in infected cells they are colocalized with GFP-SKD1(E235Q) in the E235Q compartments (arrows in D-F). The integrity of TGN structures was confirmed with -adaptin staining (G,H). Asterisks in A, D, G and H show the GFP-SKD1(E235Q)-expressing cells. Bars, 20 µm.

Return to article