Fig. 10 Negatively stained and thin section electron microscopy of PCTVs formed in the presence of Sar1-depleted cytosol and immunoelectron microscopy of these PCTVs using syntaxin 5 and rBet1 antibodies. PCTVs were formed in Sar1-immunodepleted cytosol and isolated. (A) The PCTVs were processed as in Fig. 1A. Intact vesicles ranging from 150 to 350 nm are seen. Bar, 500 nm. (B) The PCTVs were examined by thin section electron microscopy as in Fig. 1B. The PCTV were 300-350 nm in diameter. Bar, 200 nm. (C) Negatively stained PCTVs as in Fig. 1A were incubated with mouse anti-rBet1 (10 nm gold particles) and rabbit anti-syntaxin-5 (15 nm gold particles) antibodies. Representative views are shown in i-iii. In iv, pre-immune IgG was used. For details, see Materials and Methods.

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