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Fig. 11. The effect of GTP{gamma}S and H89 on PCTV and protein vesicle budding. 500 µg ER protein was incubated with (+) or without (-) cytosol or cytosol to which GTP{gamma}S (200 µM) or H89 (200 µM) was added as indicated in the key. (A) The total 14C-TAG-dpm of each 0.5 ml fraction was determined. (B) The total 3H-TCA precipitable protein-dpm was measured in each 0.5 ml fraction.





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