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First published online September 12, 2003

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Cyclin B2 - Bud3 marks the spot

In budding yeast, six B-type cyclins can associate with the single cyclin-dependent kinase (CDK) that drives the cell cycle (Cdc28). These are thought to commit it to particular tasks, although the basis for this remains elusive. They could alter CDK substrate specificity, but regulation of its subcellular localization is equally likely. Marie-Noëlle Simon and co-workers have therefore investigated the subcellular localization of Clb2, a B-type cyclin particularly important for the G2/M transition (see p. 4119). Tracking GFP fusion proteins expressed at physiological levels, they observe that Clb2 localizes to the nucleus, mitotic spindle, spindle pole bodies and - in contrast to other B-type cyclins - the mother-bud neck. Localization to the mother-bud neck requires the conserved hydrophobic patch in the protein, as well as upstream Clb2-specific sequences. Moreover, it requires the axial determinant Bud3, which the authors show colocalizes with Clb2 and interacts with it in two-hybrid assays. Since the absence of Clb2 at the mother-bud neck in bud3 mutants is associated with a delay in cytokinesis, these experiments reveal not only a novel function of Bud3 but also a possible role for neck-localized Clb2-Cdc28 in control of cytokinesis.

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Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck

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