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Fig. 2. The effects of UNC-78 and UNC-60B on the kinetics of light scattering of F-actin. F-actin (5 µM) was mixed with buffer (a, black circles), UNC-78 (1 µM) (b, white circles), UNC-60B (5 µM) (c, black triangles), or both UNC-78 and UNC-60B (d, black triangles) and the light scattering intensity (arbitrary units) was monitored over time. Time 0 was when monitoring started, which is after
20 sec of manual preparation of the samples and setting them in the instrument.