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Fig. 4. Effects of UNC-78 and UNC-60B on actin turnover as measured by nucleotide exchange. (A) F-actin (5 µM) was mixed with UNC-78 and/or UNC-60B as indicated in the figure in the presence of 40 µM {epsilon}ATP, and the fluorescence (arbitrary units) of {epsilon}ATP was monitored over time. (B) The data were fitted to exponential curves and the rates of increase in the fluorescence ({Delta}F/second) were calculated and plotted as a function of concentration of UNC-60B. Data shown are mean±s.d. of four experiments.





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