Fig. 5. mks¹ enhances polo¹ centrosomal defects. The columns display mitotic spindles from neuroblasts of the respective genotypes wild-type, polo¹, mks¹ and polo¹ mks¹ stained to reveal DNA (blue) and microtubules (green). The first three rows show immunostaining to reveal the indicated centrosomal antigens CNN, -tubulin and CP190 (all in red). The final row displays immunostaining of the mitotic phosphoepitope MPM2 (red). In all the indicated genotypes, the core centrosomal antigen CNN is localized in a wild-type manner to the centrosomes. -Tubulin and CP190 are localized to centrosomes and have a wild-type pattern of localization in both polo¹ and mks¹. However, in the double mutant, they are dispersed throughout the cell. The MPM2 epitope is present throughout the cell, at kinetochores and spindle poles in all indicated genotypes except polo¹ mks¹, in which it is absent from the spindle poles. Scale bar, 10 µm.

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