Fig. 3. Confocal images of PGs1 localisation in mouse neural cells in primary culture. (A-C) Mouse brain neurones were cultured for 2 (A), 8 (B) and 13 (C) days, then fixed with paraformaldehyde and double stained with L83 (left panels, green) and GT335 (middle panels, red). Right panels present the corresponding merged images. (D,E), Mouse brain neural cells fixed with paraformaldehyde and double stained with L83 (left panels in D and E, green) and GT335 (middle panel in D, red) or anti--tubulin mAb GTU-88 (middle panel in E, red). Right panels show the corresponding merged images. Staining of PGs1 was observed at the base of primary cilia (arrowheads) as well as of multiple cilia emerging from ependymal cells (arrows). Note also some PGs1 staining along the cilia. Scale bars: 20 µm.

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