Fig. 8. Localisation of PGs1 is dependent on the integrity of the MT network. 1009 cells were incubated with (B) or without (A) 10 µM nocodazole for 45 minutes. After washing off nocodazole, MTs were allowed to regrow for 3 minutes (C) or 6 minutes (D). (E) 1009 cells were incubated with 5 µM taxol for 45 minutes. Methanol-fixed cells were double stained with L83 (left panels in A-E) and DM1A (right panels in A-E). Scale bars: 10 µm. (F-H) Confocal green fluorescence images of living 1009/PGs1-GFP cells before (F) and after incubation with 10 µM nocodazole for 45 minutes (G) and after 10 minutes of regrowth (H).

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