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Fig. 9. PGs1 disappears from centrosomes at mitosis. (A,B) Fixed 1009/PGs1-GFP cells immunolabelled with CTR-453 (middle panels). Upper panels show the green fluorescence of PGs1-GFP and lower panels DAPI staining of DNA. The intensity of labelled cells in interphase (I), metaphase (M) and anaphase (A) is compared. (C) Time-lapse video microscopy of 1009/PGs1-GFP cells. Phase contrast (left panels) and green fluorescence (right panels) images were taken at 10 minutes intervals. Arrows indicate a cell that goes through mitosis during the time course of the experiment.





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