Fig. 2. Three-dimensional reconstruction of GFP cellular fluorescence in the outer cortex of a one-month-old TgN(GFPU)5Nagy hemizygous mouse lens. (A) Viewed end on, the variegated expression of GFP in superficial fiber cells results in a checkerboard fluorescence pattern (arrows) due to the interposition of nonexpressing cells between GFP-expressing cells. In the deeper cell layers the fluorescence is uniform (*). (B) By rotating the volume, the elongated morphology of the fiber cells becomes evident. Note the sharp transition (arrow) between the discrete labeling pattern of individual fibers in the superficial tissue and the homogeneous fluorescence of cells in the deep cortex. (C) GFP-expressing cells are uniformly fluorescent along their lengths, although regions of the fibers that contact the surface of the reconstructed volume appear somewhat brighter. (D) Viewed from beneath; the cytoplasmic fluorescence in the fibers is uninterrupted. At this depth (approximately 125 µm), all fibers contain GFP. Bar, 100 µm

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