Fig. 4. The Arm repeats are necessary for the nucleocytoplasmic shuttling of p120. (A) Individual Arm repeats were deleted in p120-3A. The mutant constructs were transduced into A431D cells and their distribution examined by immunofluorescence. Pictures shown are representative of the localization seen in the majority of cells. Deletion of Arm repeats 3 and 5 abolished nuclear localization of p120 and could not be rescued with LMB incubation. However, deletion of Arm 8 enhanced nuclear localization of p120-3A with little further effect of LMB. (B) The distribution of p120-3A deletion mutants before and after LMB treatment was scored by counting random fields of cells (200 cells per slide), and the results are indicated by the bar graphs (n=1). NC, nuclear and cytoplasmic; PC, predominantly cytoplasmic; PN, predominantly nuclear.

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