Fig. 6. p120 Arm repeats necessary for E-cadherin binding overlap with those necessary for nuclear import. (A) p120 deletion mutants are compared with respect to E-cadherin binding and nuclear localization. + indicates that the deletion mutant can bind E-cadherin and/or localize in the nucleus; ± indicates diminished steady-state levels in the nucleus; and - indicates lack of binding to E-cadherin and/or localization in the nucleus. The data show that Arm repeats 3, 4, 5 and 7 are necessary for both E-cadherin binding and nuclear localization. (B) E-cadherin-positive A431 cells were transduced with Arm 3 and Arm 5 deletion mutants of p120-3A and their distribution compared by immunofluorescence to that of E-cadherin. Both Arm 3 and Arm 5 p120 deletion mutants are found in the cytoplasm; they do not colocalize with E-cadherin, nor do they traffic into the nucleus.

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