Fig. 8. p120 nucleocytoplasmic shuttling is modulated by interactions with the microtubule system. (A) A431D cells transduced with p120-3A were double labeled with p120 (i) and -tubulin (ii) antibodies and visualized by conventional immunofluorescent microscopy. p120 colocalized with the microtubules, particularly at the nuclear periphery. (B) A431D cells transduced with p120-1A were double labeled with p120 and -tubulin antibodies before (i, ii) and after (iii, iv) LMB treatment, and visualized at the plane of the nucleus by deconvolution microscopy. Incubation with LMB shifted p120 staining from the microtubules to the nucleus, whereas tubulin staining was unaffected by LMB. The incidence of perinuclear staining of p120 was quantified by counting random fields of cells before and after LMB treatment (v). (C) A431D cells transduced with p120-1A were double labeled with p120 and -tubulin antibodies before (i, ii) and after (iii, iv) nocodazole treatment. Disruption of the microtubule network with nocodazole caused nuclear accumulation of p120-1A (iii) but not tubulin (iv). (D) p120 and microtubules were localized in p120-3A-infected A431D cells before (i, ii) and after (iii, iv) treatment with the microtubule-stabilizing drug taxol. P120-3A is strongly nuclear before taxol treatment but leaves the nucleus to associate with microtubules after stabilization of these structures by taxol.

Return to article