Merotelic kinetochore orientation occurs frequently during early mitosis in mammalian tissue cells and error correction is achieved by two different mechanisms
J Cell Sci
Cimini et al. 116 (20): 4213.
JCS00716 Supplemental Data
Files in this Data Supplement:
Table S1 -
Frequencies of merotelic orientations 'per cell' and 'per kinetochore' in different experimental conditions.
Movie 1
-
PtK1 cell
microinjected with Mad2DC during
recovery from a nocodazole-induced mitotic block. The tip of the microinjection
needle is visible in the first frame. Phase contrast pictures where acquired
every 30 seconds. Each frame is played for 1/10th of a second. The cell enters
anaphase precociously and displays two lagging chromosomes. Still images of
this movie are shown in Fig. 1D.
Movie 2
-
A cell
entering anaphase with no lagging chromosomes.PtK1
cell expressing GFP-g-tubulin.
Phase contrast is shown in green and GFP-g-tubulin
is shown in red. Phase contrast and
fluorescence images were acquired almost simultaneously every 2 minutes, using
a 4´4 binning. Each frame is played for 1/10th
of a second. Note that the centrosomes (red) are well separated and localized
on opposite sides of the nucleus when the nuclear envelope breaks down.
Movie 3
-
This cell
shows a chromosome lagging behind when all the others migrate to the spindle
poles in anaphase. PtK1 cell expressing GFP-g-tubulin. Phase contrast is shown in green and GFP-g-tubulin is shown in red. Phase contrast and fluorescence images were
acquired almost simultaneously every 2 minutes, using a 4´4 binning. Each frame is played for 1/10th
of a second. Note, in this cell centrosomes (red) move apart to opposite sides
of the nucleus before nuclear envelope breakdown.
