Fig. 6. PtK1 cells in early mitosis immunostained for CREST and -tubulin (left column). DNA staining by DAPI is shown in the right column. (A,A',B,B' Cells fixed immediately after nuclear envelope breakdown. Two forming microtubule organizing centers can be seen (arrows) in these cells. The CREST staining shows that kinetochores are distributed around the spindle poles and are oriented in many different directions with respect to the poles (B). Furthermore, some chromosomes are oriented with their centromere axis through sister kinetochores nearly parallel to the spindle axis (yellow arrowhead in A), whereas other sister pairs are nearly perpendicular (white arrowheads in A). (C,C') Prometaphase control cell. Only two microtubule organizing centers can be identified (spindle poles) in this cell, as in the vast majority of untreated PtK1 mitotic cells. Scale bar: 10 µm.

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