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Fig. 7. Effect of a prolonged metaphase on the presence of anaphase lagging chromosomes. (A) Both control PtK1 cells (CTRL) and cells recovering from a nocodazole (NOC)-induced mitotic block were either fixed untreated or delayed in metaphase by a 2-hour MG-132 treatment and then fixed after recovery. Anaphase lagging chromosomes were analyzed by CREST staining. N, total number of cells analyzed. (B) Frequencies of single and multiple lagging chromosomes in anaphase PtK1 cells recovering from a nocodazole-induced mitotic block (-MG-132), or in which anaphase onset was delayed by a 2-hour MG-132 treatment (+MG-132) during recovery from nocodazole.





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