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Fig. 6. Replication-defective adenovirus induced expression of GFP-myosin in C2C12 myotubes. Post-mitotic C2C12 myocytes were infected ~48 hours after transfer to fusion medium and maintained in culture for an additional 4 days. Almost all of the large myotubes are brightly fluorescent from the expressed GFP-myosin. When viewed at higher magnification (inset) it is clear that the WT GFP-myosin has assembled with the endogenous C2C12 myosin into ordered striated myofibrils.





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