Fig. 8. The myosin concentration dependence of the actin filament velocity measured in an in vitro motility assay. (A) The velocity of actin filament was measured on antibody capture surfaces incubated with various concentrations of WT and G584R myosin. The velocity of sliding movement at saturating myosin surface density is indicated. (B) The R403Q and R453C data are plotted and the actin filament velocity is indicated. The data points correspond to the mean (±s.d.) for a Gaussian distribution of the actin filament velocity at each myosin concentration tested (Kinose et al., 1996). The myosin concentration dependence of the actin filament sliding velocity was fit to the empirical equation: v=v_max(1-e^-m(-_onset)). The GFP-myosin concentration was determined by densitometry of stained SDS gels and fluorescence spectroscopy. Actin filament motility was measured at 30°C.

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