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Fig. 4. Dsg3dim population of palm keratinocytes survived better than Dsg3bri population in long-term culture. (A) Phase-contrast images (a,b; arrows delineate margin of colony) of different clone types in Dsg3dim and Dsg3bri at passage 2. Large colonies are observed in the Dsg3dim (a) but not the Dsg3bri population, in which almost all the colonies are abortive (b). (B) Assessment of the frequency of abortive colonies in Dsg3dim and Dsg3bri at passage 2. Of 96 colonies in Dsg3dim only 59 are abortive colonies, however, 32 out of 33 are abortive colonies in Dsg3bri (P<0.05). Scale bar: (in A) 50 µm.