Fig. 6. Keratinocytes in Dsg3^dim orß1^bri/Dsg3^dim populations have a greater overall proliferative potential than cells in the Dsg3^bri or ß1^bri/Dsg3^bri populations. (A) Passage number and (B) duration of cell culture of different keratinocyte fractions in six independent experiments. The age of the skin donor was between 15 and 50 years old. Cells used for FACS were all from passage 1 following initial cultivation from the tiny biopsies. At each passage, cells were plated at the same density for each fraction. Note both the passage number and culture duration show significant differences (^*P<0.05), and cells in Dsg3^dim or ß1^bri/Dsg3^dim populations are capable of expanding more passages and surviving for a longer periods in culture than cells in the Dsg3^bri or ß1^bri/Dsg3^bri populations.

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