Fig. 1A. Depolarization- and InsP₃-evoked [Ca²⁺]_c increases in myocytes. (Aa) Depolarization to +10 mV (from –70 mV) (v) activated I_Ca (iv) and increased [Ca²⁺]_c as represented by the intensity of colour changes in the frames (ii) and fluorescence traces (F/F₀; iii). The cell [Ca²⁺]_c images in the frames in (ii) were taken before (A), during (B,C) and after (D-F) the depolarization, and show the [Ca²⁺]_c changes occurring throughout the cell. The precise times at which the images were obtained are indicated by their respective letters above the Ca²⁺ line traces in (ii). The fluorescence ratio (F/F₀) changes plotted against time (iii) come from 1 pixel wide lines (536 nm) across the cell at 10 µm intervals. The positions of the lines are indicated in (i) (right-hand panel), although the lines drawn are wider than 1 pixel to facilitate visualization. A bright-field image of the cell and position of the patch pipette is shown (i, left-hand panel). (Ab) Similar parameters were measured in the same cell after photolysis of caged InsP₃ () which also increased [Ca²⁺]_c (ii,iii).

Return to article