JCS00726 Movie

Files in this Data Supplement:

• Movie 1 - Ax-2 cells were submitted to a 1 Pa shear stress in Sörensen buffer and monitored for 10 minutes under dark field illumination at a 2.5x magnification. The hydrodynamic flow is directed to the right and starts after the first image. A set of representative cell tracks is shown. The size of an image is 1.2x0.9 mm. Images are separated by 30 seconds.
• Movie 2 - Ax-2 cells were submitted to a 1 Pa shear stress in Sörensen buffer and 20 mg/ml N-(3-chlorophenyl)-isopropyl-carbamate (CIPC), and monitored for 10 minutes under dark field illumination at a 2.5x magnification. The hydrodynamic flow is directed to the right and starts after the first image. A set of representative cell tracks is shown. The size of an image is 1.2x0.9 mm. Images are separated by 30 seconds.
• Movie 3 - Ax-2 cells were resuspended in Sörensen buffer and allowed to settle for 3 minutes in the lateral flow chamber. The cells were observed by phase-contrast microscopy at a 40x magnification. Recording was started and, after 30 seconds, a constant shear flow (s=2 Pa) was applied to the right (arrow) for 60 seconds. The size of an image is 105x67 mm. Images are separated by 3 seconds. Pseudopods are seen as dark extensions of the side of the cells. Upon onset of the flow, one cell rapidly detaches while the others emit new pseudopods in the direction of the flow.
• Movie 4 - Ax-2 cells were submitted to a 1 Pa shear stress in Sörensen buffer and 34 mM LY294002, and monitored for 10 minutes under dark field illumination at a 2.5x magnification. The hydrodynamic flow is directed to the right and starts after the first image. A set of representative cell tracks is shown. The size of an image is 1.2x0.9 mm. Images are separated by 30 seconds.